MYCOBACTERİUM TUBERCULOSİS KOMPLEKSİ KLİNİK İZOLATLARINDA İZONİAZİD VE RİFAMPİSİN DİRENCİNİN HIZLI TANISI İÇİN ‘REVERSE BLOT HYBRIDIZATION ASSAY MYCOBACTERIUM TUBERCULOSIS DRUG RESISTANCE’ YÖNTEMİNİN ETKİNLİĞİNİN ARAŞTIRILMASI
نویسندگان
چکیده
منابع مشابه
Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection.
Direct smear examination using Ziehl-Neelsen staining for pulmonary tuberculosis (PTB) diagnosis is inexpensive and easy to use, but has the major limitation of low sensitivity. Rapid molecular methods are becoming more widely available in centralized laboratories, but they depend on timely reporting of results and strict quality assurance obtainable only from costly commercial kits available i...
متن کاملA STUDY OF MYCOBACTERIUM TUBERCULOSIS DRUG RESISTANCE IN PULMONARY TUBERCULOSIS
Tuberculosis remains a major public health problem in both developing and developed countries. Drug-resistant tuberculosis is an increasing health problem and serious challenge to tuberculosis (TB) control programs. Information about the susceptibility pattern of Mycobacterium tuberculosis isolates against anti-tuberculosis drugs is an important aspect to TB control. The objectives of the s...
متن کاملRapid detection of rifampin-resistant clinical isolates of Mycobacterium tuberculosis by reverse dot blot hybridization.
OBJECTIVE A PCR-reverse dot blot hybridization (RDBH) assay was developed for rapid detection of rpoB gene mutations in 'hot mutation region' of Mycobacterium tuberculosis (M. tuberculosis). METHODS 12 oligonucleotide probes based on the wild-type and mutant genotype rpoB sequences of M. tuberculosis were designed to screen the most frequent wild-type and mutant genotypes for diagnosing RIF r...
متن کاملReplacing reverse line blot hybridization spoligotyping of the Mycobacterium tuberculosis complex.
Spoligotyping is a tool for the molecular characterization/typing of Mycobacterium tuberculosis complex (MTBC) strains based on target sequences (spacers) in the direct repeat (DR) region (14). The standard spoligotyping assay involves the hybridization of amplified sample DNA to nylon membrane-immobilized oligonucleotides whose sequences are representative of 43 spacer regions. Variations in t...
متن کاملMultiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Multiplex PCR/Reverse Line Blot Hybridization assay allows the detection of up to 43 molecular targets in 43 samples using one multiplex PCR reaction followed by probe hybridization on a nylon membrane, which is re-usable. Probes are 5' amine modified to allow fixation to the membrane. Primers are 5' biotin modified which allows detection of hybridized PCR products using streptavidin-peroxidase...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: SDÜ Tıp Fakültesi Dergisi
سال: 2021
ISSN: 1300-7416
DOI: 10.17343/sdutfd.534941